TACC3 on microtubule plus ends

TACC3 on microtubule plus ends

Hippocampal neurons

Hippocampal neurons

Chromosomes and ER in mitosis

Chromosomes and ER in mitosis

Transferrin stuck on the cell surface in mitosis

Transferrin stuck on the cell surface in mitosis

Hippocampal neurons

Hippocampal neurons

Neuronal microisland

Neuronal microisland

The Digital Cell

The Digital Cell

Papers

  • A geometrically informed permutation test for dependency in spatiotemporal patterns of protein species in microscopy images
    Honnor, T.R., Royle, S.J., Johansen, A.M., Brettschneider, J.A.
    J Theor Biol (2026) 624, 112409
    Details Paper
  • Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interaction
    Gunning, V, Batchelor, M, Alexander, KK, Walko, M, Dill, T.C, Burgess, SG, Royle, SJ, Kennedy, EJ, Bayliss, R
    Structure (2026) 34, 915-931.e12
    Details Paper Preprint
  • The GLUT4 storage vesicle pool is maintained by intracellular nanovesicle traffic
    Courthold, E., Larocque, G., Royle, S.J.
    bioRxiv (2026)
    Details Paper Preprint Data/Code
  • ATG9A vesicles are a subtype of intracellular nanovesicle
    Fesenko M, Moore DJ, Ewbank P, Courthold, E, Royle SJ.
    J Cell Sci (2025) 138, jcs263852
    Details Paper Preprint Data/Code
  • Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor
    Downie L, Ferrandiz N, Courthold E, Jones M, Royle SJ.
    Plos Biol (2025) 23, e3003249
    Details Paper Preprint Data/Code
  • Structural characterization and inhibition of the interaction between ch-TOG and TACC3
    Shelford J, Burgess SG, Rostkova E, Richards MW, Larocque, G, Sampson, J, Tiede C, Fielding AJ, Daviter T, Tomlinson DC, Calabrese AN, Pfuhl M, Bayliss R, Royle SJ.
    J Cell Biol (2025) 224, e202407002
    Details Paper Preprint Data/Code
  • Hotwiring integrin endocytosis acutely modulates cell interactions
    Kamboj S, Boché A, Moret A, Wang Z, Aimé C, Agniel R, Leroy-Dudal J, Carreiras F, Gallet O, Royle SJ, Lambert A
    bioRxiv (2024)
    Details Paper Preprint
  • Passive diffusion accounts for the majority of intracellular nanovesicle transport
    Sittewelle M, Royle SJ.
    Life Sci Alliance (2024) 7, e202302406
    Details Paper Preprint Data/Code
  • Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes
    Ferrandiz N, Downie L, Starling GP, Royle SJ.
    J Cell Biol (2022) 221, e202203021
    Details Paper Preprint Data/Code
  • Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation
    Küey C, Sittewelle M, Larocque G, Hernández-González M, Royle SJ.
    Elife (2022) 11, e78929
    Details Paper Preprint Data/Code
  • Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization
    Ryan EL, Shelford J, Massam-Wu T, Bayliss R, Royle SJ.
    J Cell Sci (2021) 134, jcs255794
    Details Paper Preprint Data/Code
  • Intracellular nanovesicles mediate α5β1 integrin trafficking during cell migration
    Larocque G, Moore DJ, Sittewelle M, Kuey C, Hetmanski JHR, La-Borde PJ, Wilson BJ, Clarke NI, Caswell PT, Royle SJ.
    J Cell Biol (2021) 220, e202009028
    Details Paper Preprint Data/Code
  • Multi-modal adaptor-clathrin contacts drive coated vesicle assembly
    Smith SM, Larocque G, Wood KM, Morris KL, Roseman AM, Sessions RB, Royle SJ, Smith CJ.
    EMBO J (2021) 40, e108795
    Details Paper Preprint
  • Tumor protein D54 defines a new class of intracellular transport vesicles
    Larocque G, La-Borde PJ, Clarke NI, Carter NJ, Royle SJ.
    J Cell Biol (2020) 219, e201812044
    Details Paper Preprint Data/Code
  • Calculating the maximum capacity of intracellular transport vesicles
    Martins Ratamero E, Royle SJ
    bioRxiv (2019)
    Details Paper Preprint
  • Unintended perturbation of protein function using GFP nanobodies in human cells
    Küey C, Larocque G, Clarke NI, Royle SJ.
    J Cell Sci (2019) 132, jcs234955
    Details Paper Preprint
  • FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy
    Clarke NI, Royle SJ.
    Nat Commun (2018) 9, 2604
    Details Paper Preprint Data/Code
  • Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix
    Burgess SG, Mukherjee M, Sabir S, Joseph N, Gutiérrez-Caballero C, Richards MW, Huguenin-Dezot N, Chin JW, Kennedy EJ, Pfuhl M, Royle SJ, Gergely F, Bayliss R.
    EMBO J (2018) 37,
    Details Paper
  • Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment
    Auckland P, Clarke NI, Royle SJ, McAinsh AD.
    J Cell Biol (2017) 216, 1623–1639
    Details Paper
  • FGFR3-TACC3 cancer gene fusions cause mitotic defects by removal of endogenous TACC3 from the mitotic spindle
    Sarkar S, Ryan EL, Royle SJ.
    Open Biol (2017) 7, rsob.170080
    Details Paper Data/Code
  • Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis
    Nixon FM, Honnor TR, Clarke NI, Starling GP, Beckett AJ, Johansen AM, Brettschneider JA, Prior IA, Royle SJ.
    J Cell Sci (2017) 130, 1845–1855
    Details Paper Preprint
  • New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision
    Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ.
    J Cell Biol (2017) 216, 4351-4365
    Details Paper Preprint Data/Code
  • A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development
    Nahorski MS, Al-Gazali L, Hertecant J, Owen DJ, Borner GH, Chen YC, Benn CL, Carvalho OP, Shaikh SS, Phelan A, Robinson MS, Royle SJ, Woods CG.
    Brain (2015) 138, 2147–2160
    Details Paper
  • Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression
    O'Regan L, Sampson J, Richards MW, Knebel A, Roth D, Hood FE, Straube A, Royle SJ, Bayliss R, Fry AM.
    J Cell Biol (2015) 209, 349-358
    Details Paper
  • TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
    Gutiérrez-Caballero C, Burgess SG, Bayliss R, Royle SJ.
    Biol Open (2015) 4, 170-179
    Details Paper Preprint
  • The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
    Nixon FM, Gutiérrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle SJ.
    Elife (2015) 4, e07635
    Details Paper
  • An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis
    Kaur S, Fielding AB, Gassner G, Carter NJ, Royle SJ.
    Elife (2014) 3, e00829
    Details Paper Preprint
  • Non-specificity of Pitstop 2 in clathrin-mediated endocytosis
    Willox AK, Sahraoui YM, Royle SJ.
    Biol Open (2014) 3, 326-331
    Details Paper Preprint
  • Presynaptic clathrin levels are a limiting factor for synaptic transmission
    López-Murcia FJ, Royle SJ, Llobet A.
    J Neurosci (2014) 34, 8618–8629
    Details Paper
  • Coordination of adjacent domains mediates TACC3-ch-TOG-clathrin assembly and mitotic spindle binding
    Hood FE, Williams SJ, Burgess SG, Richards MW, Roth D, Straube A, Pfuhl M, Bayliss R, Royle SJ.
    J Cell Biol (2013) 202, 463–478
    Details Paper
  • Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension
    Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ.
    J Cell Sci (2013) 126, 2102–2113
    Details Paper
  • Clathrin-mediated endocytosis is inhibited during mitosis
    Fielding AB, Willox AK, Okeke E, Royle SJ.
    Proc Natl Acad Sci U S A (2012) 109, 6572-6577
    Details Paper
  • Constitutive clathrin-mediated endocytosis of CTLA-4 persists during T cell activation
    Qureshi OS, Kaur S, Hou TZ, Jeffery LE, Poulter NS, Briggs Z, Kenefeck R, Willox AK, Royle SJ, Rappoport JZ, Sansom DM.
    J Biol Chem (2012) 287, 9429–9440
    Details Paper
  • Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain
    Willox AK, Royle SJ.
    Traffic (2012) 13, 70-81
    Details Paper
  • Stonin 2 is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval
    Willox AK, Royle SJ.
    Curr Biol (2012) 22, 1435–1439
    Details Paper
  • A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging
    Booth DG, Hood FE, Prior IA, Royle SJ.
    EMBO J (2011) 30, 906–919
    Details Paper
  • Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges
    Cheeseman LP, Booth DG, Hood FE, Prior IA, Royle SJ.
    Commun Integr Biol (2011) 4, 409–412
    Details Paper
  • Clathrin heavy chain gene fusions expressed in human cancers: analysis of cellular functions
    Blixt MK, Royle SJ.
    Traffic (2011) 12, 754–761
    Details Paper
  • Comment on "The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles"
    Granseth B, Odermatt B, Royle SJ, Lagnado L.
    Science (2009) 325, 1499
    Details Paper
  • Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis
    Hood FE, Royle SJ.
    J Cell Sci (2009) 122, 2185–2190
    Details Paper
  • Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses
    Granseth B, Odermatt B, Royle SJ, Lagnado L.
    Neuron (2006) 51, 773–786
    Details Paper
  • Trimerisation is important for the function of clathrin at the mitotic spindle
    Royle SJ, Lagnado L.
    J Cell Sci (2006) 119, 4071–4078
    Details Paper
  • Clathrin is required for the function of the mitotic spindle
    Royle SJ, Bright NA, Lagnado L.
    Nature (2005) 434, 1152–1157
    Details Paper
  • Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors
    Royle SJ, Qureshi OS, Bobanović LK, Evans PR, Owen DJ, Murrell-Lagnado RD.
    J Cell Sci (2005) 118, 3073–3080
    Details Paper
  • Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor
    Royle SJ, Bobanović LK, Murrell-Lagnado RD.
    J Biol Chem (2002) 277, 35378–35385
    Details Paper
  • P2X receptor trafficking in neurons is subunit specific
    Bobanovic LK, Royle SJ, Murrell-Lagnado RD.
    J Neurosci (2002) 22, 4814–4824
    Details Paper
  • Behavioural analysis and susceptibility to CNS injury of four inbred strains of mice
    Royle SJ, Collins FC, Rupniak HT, Barnes JC, Anderson R.
    Brain Res (1999) 816, 337-349
    Details Paper
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Reviews & Methods

  • The Secret (Mitotic) Life of Clathrin
    Royle SJ.
    Traffic (2026) 27, e70047
    Details Paper
  • Small but mighty: ATG9A-positive vesicles are a branch of the intracellular nanovesicle superfamily
    Fesenko M, Royle SJ.
    Autophagy Rep (2025) 4, 2513467
    Details Paper
  • 3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM)
    Ferrandiz N, Royle SJ.
    Bio Protoc (2023) 13, e4708
    Details Paper
  • Genetically encoded imaging tools for investigating cell dynamics at a glance
    Sittewelle M, Ferrandiz N, Fesenko M, Royle SJ.
    J Cell Sci (2023) 136, jcs260783
    Details Paper
  • Integrating intracellular nanovesicles into integrin trafficking pathways and beyond
    Larocque G, Royle SJ.
    Cell Mol Life Sci (2022) 79, 335
    Details Paper
  • The NAE Pathway: Autobahn to the Nucleus for Cell Surface Receptors
    Shah P, Chaumet A, Royle SJ, Bard FA.
    Cells (2019) 8, 915
    Details Paper
  • Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture
    Clarke NI, Royle SJ.
    Methods Cell Biol (2018) 145, 29–43
    Details Paper
  • Imaging "Hot-Wired" Clathrin-Mediated Endocytosis
    Wood LA, Royle SJ.
    Methods Mol Biol (2018) 1847, 83–94
    Details Paper
  • Super-duper resolution imaging of mitotic microtubules
    Royle SJ.
    Nat Rev Mol Cell Biol (2015) 16, 67
    Details Paper
  • Zero tolerance: amphipathic helices in endocytosis
    Wood LA, Royle SJ.
    Dev Cell (2015) 33, 119–120
    Details Paper
  • Mitotic inhibition of clathrin-mediated endocytosis
    Fielding AB, Royle SJ.
    Cell Mol Life Sci (2013) 70, 3423–3433
    Details Paper
  • Protein adaptation: mitotic functions for membrane trafficking proteins
    Royle SJ.
    Nat Rev Mol Cell Biol (2013) 14, 592–599
    Details Paper
  • Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy
    Booth DG, Cheeseman LP, Prior IA, Royle SJ.
    Methods Cell Biol (2013) 115, 327–342
    Details Paper
  • The role of clathrin in mitotic spindle organisation
    Royle SJ.
    J Cell Sci (2012) 125, 19–28
    Details Paper
  • Mitotic moonlighting functions for membrane trafficking proteins
    Royle SJ.
    Traffic (2011) 12, 791–798
    Details Paper
  • Pulling it together: The mitotic function of TACC3
    Hood FE, Royle SJ.
    Bioarchitecture (2011) 1, 105–109
    Details Paper
  • Clathrin-mediated endocytosis at the synaptic terminal: bridging the gap between physiology and molecules
    Royle SJ, Lagnado L.
    Traffic (2010) 11, 1489-1497
    Details Paper
  • Imaging phluorin-based probes at hippocampal synapses
    Royle SJ, Granseth B, Odermatt B, Derevier A, Lagnado L.
    Methods Mol Biol (2008) 457, 293–303
    Details Paper
  • Clathrin-mediated endocytosis: the physiological mechanism of vesicle retrieval at hippocampal synapses
    Granseth B, Odermatt B, Royle SJ, Lagnado L.
    J Physiol (2007) 585, 681–686
    Details Paper
  • The cellular functions of clathrin
    Royle SJ.
    Cell Mol Life Sci (2006) 63, 1823–1832
    Details Paper
  • Constitutive cycling: a general mechanism to regulate cell surface proteins
    Royle SJ, Murrell-Lagnado RD.
    Bioessays (2003) 25, 39-46
    Details Paper
  • Endocytosis at the synaptic terminal
    Royle SJ, Lagnado L.
    J Physiol (2003) 553, 345–355
    Details Paper
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Other publications

  • The Company of Biologists: celebrating 100 years
    Bray SJ, Royle SJ, Shiels HA, St Johnston, D
    J Cell Sci (2025) 138, jcs263761
    Details Paper
  • Recommendations for accelerating open preprint peer review to improve the culture of science
    Avissar-Whiting M, Belliard F, Bertozzi SM, Brand A, Brown K, Clément-Stoneham G, Dawson S, Dey G, Ecer D, Edmunds SC, Farley A, Fischer TD, Franko M, Fraser JS, Funk K, Ganier C, Harrison M, Hatch A, Hazlett H, Hindle S, Hook DW, Hurst P, Kamoun S, Kiley R, Lacy MM, LaFlamme M, Lawrence R, Lemberger T, Leptin M, Lumb E, MacCallum CJ, Marcum CS, Marinello G, Mendonça A, Monaco S, Neves K, Pattinson D, Polka JK, Puebla I, Rittman M, Royle SJ, Saderi D, Sever R, Shearer K, Spiro JE, Stern B, Taraborelli D, Vale R, Vasquez CG, Waltman L, Watt FM, Weinberg ZY, Williams M.
    PLoS Biol (2024) 22, e3002502
    Details Paper
  • Comparing antibodies for the detection of ch-TOG/CKAP5 in mitotic HeLa cells by indirect immunofluorescence
    Shelford J, Royle SJ
    Figshare (2020)
    Details
  • The Digital Cell: Cell biology as a data science
    Royle SJ
    Cold Spring Harbor Laboratory Press (2020)
    Details
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