Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges

paper

mitosis

Author

Cheeseman LP, Booth DG, Hood FE, Prior IA, Royle SJ.

Doi

https://doi.org/10.4161/cib.4.4.15250

Citation

Cheeseman, L.P., Booth, D.G., Hood, F.E., Prior, I.A., and Royle, S.J. (2011). Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges. Commun Integr Biol 4, 409–412.

Abstract

Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.